CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES

Kuan Yan, Fons Verbeek, Sylvia Le Dévédec, Bob van de Water

2009

Abstract

In this paper, we address the problem of the analysis of cellular phenotype from time-lapse image sequences using object tracking algorithms and feature extraction and classification. We discusses the application of an object tracking algorithm for in the analysis of high content cell-migration time-lapse image sequence of extremely motile cells; these cells are captured at low time-resolution.. The small size of the objects and significant deformation of the object during the process renders the tracking as a non-trivial problem. To that end, the ‘KDE Mean Shift’, a real-time tracking solution, is adapted for our research. We illustrate that in a simulation experiment with artificial objects, with our algorithm an accuracy of over 90% can be established. Based on the tracking result, we propose several morphology and motility based measurements for the analysis of cell behaviour. Our analysis requires only initial manual interference; the majority of the processing is automated.

Paper Citation

in Harvard Style

Yan K., Verbeek F., Le Dévédec S. and van de Water B. (2009). CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES . In Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009) ISBN 978-989-8111-69-2, pages 281-286. DOI: 10.5220/0001804302810286

in Bibtex Style

@conference{visapp09,
author={Kuan Yan and Fons Verbeek and Sylvia Le Dévédec and Bob van de Water},
title={CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES },
booktitle={Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009)},
year={2009},
pages={281-286},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0001804302810286},
isbn={978-989-8111-69-2},
}

in EndNote Style

TY - CONF
JO - Proceedings of the Fourth International Conference on Computer Vision Theory and Applications - Volume 1: VISAPP, (VISIGRAPP 2009)
TI - CELL TRACKING AND DATA ANALYSIS OF IN VITRO TUMOUR CELLS FROM TIME-LAPSE IMAGE SEQUENCES
SN - 978-989-8111-69-2
AU - Yan K.
AU - Verbeek F.
AU - Le Dévédec S.
AU - van de Water B.
PY - 2009
SP - 281
EP - 286
DO - 10.5220/0001804302810286