Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics

Silvia Mittler

2016

Abstract

Two evanescent field microscopy technologies based on glass slab waveguides with permanent coupling gratings are presented: waveguide evanescent field fluorescence microscopy (WEFF) and waveguide evanescent field scattering microscopy (WEFS). The technologies are briefly described: the experimental setup is based on a conventional inverted microscope. A comparison to TIR and TIRF microscopy is given. The advantages of the waveguide method are clearly addressed. Various examples from for WEFF and WEFS microscopy are given. For WEFF: static distance mapping with a multimode waveguide, dynamic solubilisation studies of cell plasma membranes and the kinetic response of osteoblasts to trypsin. For WEFS: bacteria sterilization as well as cell adhesion and granularity studies. The latest development is a mass producible all-polymer-waveguide-chip to bring the technology to the interested scientific community.

Paper Citation

in Harvard Style

Mittler S. (2016). Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics . In Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS, ISBN 978-989-758-174-8, pages 201-212. DOI: 10.5220/0005618302010212

in Bibtex Style

@conference{photoptics16,
author={Silvia Mittler},
title={Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics},
booktitle={Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,},
year={2016},
pages={201-212},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005618302010212},
isbn={978-989-758-174-8},
}

in EndNote Style

TY - CONF
JO - Proceedings of the 4th International Conference on Photonics, Optics and Laser Technology - Volume 1: PHOTOPTICS,
TI - Waveguide Evanescent Field Microscopies for Application in Cell- and Bacteria- Biophysics
SN - 978-989-758-174-8
AU - Mittler S.
PY - 2016
SP - 201
EP - 212
DO - 10.5220/0005618302010212