An Image Generator Platform to Improve Cell Tracking Algorithms - Simulation of Objects of Various Morphologies, Kinetics and Clustering

Pedro Canelas, Leonardo Martins, André Mora, Andre S. Ribeiro, José Fonseca

2016

Abstract

Several major advances in Cell and Molecular Biology have been made possible by recent advances in live-cell microscopy imaging. To support these efforts, automated image analysis methods such as cell segmentation and tracking during a time-series analysis are needed. To this aim, one important step is the validation of such image processing methods. Ideally, the “ground truth” should be known, which is possible only by manually labelling images or in artificially produced images. To simulate artificial images, we have developed a platform for simulating biologically inspired objects, which generates bodies with various morphologies and kinetics and, that can aggregate to form clusters. Using this platform, we tested and compared four tracking algorithms: Simple Nearest-Neighbour (NN), NN with Morphology and two DBSCAN-based methods. We show that Simple NN works well for small object velocities, while the others perform better on higher velocities and when clustering occurs. Our new platform for generating new benchmark images to test image analysis algorithms is openly available at (http://griduni.uninova.pt/Clustergen/ClusterGen_v1.0.zip).

Paper Citation

in Harvard Style

Canelas P., Martins L., Mora A., S. Ribeiro A. and Fonseca J. (2016). An Image Generator Platform to Improve Cell Tracking Algorithms - Simulation of Objects of Various Morphologies, Kinetics and Clustering . In Proceedings of the 6th International Conference on Simulation and Modeling Methodologies, Technologies and Applications - Volume 1: SIMULTECH, ISBN 978-989-758-199-1, pages 44-55. DOI: 10.5220/0005957800440055

in Bibtex Style

@conference{simultech16,
author={Pedro Canelas and Leonardo Martins and André Mora and Andre S. Ribeiro and José Fonseca},
title={An Image Generator Platform to Improve Cell Tracking Algorithms - Simulation of Objects of Various Morphologies, Kinetics and Clustering},
booktitle={Proceedings of the 6th International Conference on Simulation and Modeling Methodologies, Technologies and Applications - Volume 1: SIMULTECH,},
year={2016},
pages={44-55},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005957800440055},
isbn={978-989-758-199-1},
}

in EndNote Style

TY - CONF
JO - Proceedings of the 6th International Conference on Simulation and Modeling Methodologies, Technologies and Applications - Volume 1: SIMULTECH,
TI - An Image Generator Platform to Improve Cell Tracking Algorithms - Simulation of Objects of Various Morphologies, Kinetics and Clustering
SN - 978-989-758-199-1
AU - Canelas P.
AU - Martins L.
AU - Mora A.
AU - S. Ribeiro A.
AU - Fonseca J.
PY - 2016
SP - 44
EP - 55
DO - 10.5220/0005957800440055