AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES

P. Krämer, F. Boto, D. Wald, F. Bessy, C. Paloc, C. Callol, A. Letamendia, I. Ibarbia, O. Holgado, J. M. Virto

2010

Abstract

Embryos of animal models are becoming widely used to study cardiac development and genetics. However, the analysis of the embryonic heart is still mostly done manually. This is a very laborious and expensive task as each embryo has to be inspected visually by a biologist. We therefore propose to automatically segment the embryonic heart from high-speed fluorescence microscopy image sequences, allowing morphological and functional quantitative features of cardiac activity to be extracted. Several methods are presented and compared within a large range of images, varying in quality, acquisition parameters, and embryos position. Although manual control and visual assessment would still be necessary, the best of our methods has the potential to drastically reduce biologist workload by automating manual segmentation.

Paper Citation

in Harvard Style

Krämer P., Boto F., Wald D., Bessy F., Paloc C., Callol C., Letamendia A., Ibarbia I., Holgado O. and Virto J. (2010). AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES . In Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010) ISBN 978-989-674-018-4, pages 121-126. DOI: 10.5220/0002342501210126

in Bibtex Style

@conference{biosignals10,
author={P. Krämer and F. Boto and D. Wald and F. Bessy and C. Paloc and C. Callol and A. Letamendia and I. Ibarbia and O. Holgado and J. M. Virto},
title={AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES},
booktitle={Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)},
year={2010},
pages={121-126},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0002342501210126},
isbn={978-989-674-018-4},
}

in EndNote Style

TY - CONF
JO - Proceedings of the Third International Conference on Bio-inspired Systems and Signal Processing - Volume 1: BIOSIGNALS, (BIOSTEC 2010)
TI - AUTOMATIC SEGMENTATION OF EMBRYONIC HEART IN TIME-LAPSE FLUORESCENCE MICROSCOPY IMAGE SEQUENCES
SN - 978-989-674-018-4
AU - Krämer P.
AU - Boto F.
AU - Wald D.
AU - Bessy F.
AU - Paloc C.
AU - Callol C.
AU - Letamendia A.
AU - Ibarbia I.
AU - Holgado O.
AU - Virto J.
PY - 2010
SP - 121
EP - 126
DO - 10.5220/0002342501210126