Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization

V. Errico, R. Molinaro, C. Gargioli, F. Ferranti, M. Dinescu, S. Cannata, G. Saggio, S. Rufini, A. Desideri

2016

Abstract

The membrane-substrate interactions have a topological valence and represent a level of information exchange between the cell and the extra-cellular matrix and/or between cells. The interactions can vary with boundary conditions and can be altered by varying the chemical and/or physical properties of the substrate. The alteration can presumably result in differentiation or specialization of the cells, but this fundamental aspect must still be fully understood. In such a frame, we investigated the levels of transcriptional coactivators YAP/TAZ throughout C2C12 differentiation on standard two-dimensional substrates and on polyethylene glycol-fibrinogen three-dimensional microenvironment. In detail, we observed that the use of a three-dimensional matrix permits an earlier differentiation in muscular cells when compared to standard bidimensional substrates. On such a basis, we want to investigate the modulation of a more regular threedimensional pattern on cells proliferation response and we propose a matrix, generable with multiphoton absorption, with regular aligned channels in order to overcome the current limitation in muscle regeneration techniques, so a possible tool to improve the myofibers formation and alignment.

Paper Citation

in Harvard Style

Errico V., Molinaro R., Gargioli C., Ferranti F., Dinescu M., Cannata S., Saggio G., Rufini S. and Desideri A. (2016). Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization . In Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016) ISBN 978-989-758-170-0, pages 241-246. DOI: 10.5220/0005790402410246

in Bibtex Style

@conference{biodevices16,
author={V. Errico and R. Molinaro and C. Gargioli and F. Ferranti and M. Dinescu and S. Cannata and G. Saggio and S. Rufini and A. Desideri},
title={Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization},
booktitle={Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)},
year={2016},
pages={241-246},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0005790402410246},
isbn={978-989-758-170-0},
}

in EndNote Style

TY - CONF
JO - Proceedings of the 9th International Joint Conference on Biomedical Engineering Systems and Technologies - Volume 1: BIODEVICES, (BIOSTEC 2016)
TI - Cells Microenvironment Engineering - Multiphoton Absorption for Muscle Regeneration Optimization
SN - 978-989-758-170-0
AU - Errico V.
AU - Molinaro R.
AU - Gargioli C.
AU - Ferranti F.
AU - Dinescu M.
AU - Cannata S.
AU - Saggio G.
AU - Rufini S.
AU - Desideri A.
PY - 2016
SP - 241
EP - 246
DO - 10.5220/0005790402410246